Detection of p58⁺ T cells and CTL clone 4C7–specific TCR rearrangements by CDR3 analysis ex vivo in tumor cells and PBMC.

RNA from p58⁺ T cells, tumor, and PBMC were reverse transcribed and amplified by using a TCRBV6 or TCRBV8 and a BC primer. Amplified DNA was copied in a runoff reaction by using fluorescent BC or BJ primers. The amplified products were analyzed on an automated sequencer. The profiles obtained show the size in nt (x-axis) and fluorescence intensity (y-axis) of the amplified products. The absolute FU values are indicated by asterisks.