Analysis of T-cell receptor (TCR) BV repertoire expressed by in vitro–derived p58⁺ and p58⁻ T-cell lines and ex vivo samples of tumor and peripheral blood mononuclear cells (PBMC).

Comparison of relative expression of TCRBV gene segment in a p58⁺ TIL cell line (A), a p58⁻ TIL cell line (B), and a tumor fragment (▪) and PBMC (□) (C). For each sample, the spots obtained by Southern blotting of TCRBV gene segments amplified by polymerase chain reaction (30 cycles) were analyzed by densitometry, and each BV signal was expressed as a percentage of the sum of the values of all BV spots.