Fig. 6.
Fig. 6. Light microscopy of BCBL-1 cells treated with TPA plus hr-NGF or anti-NGF Ab and observed at 48 hours of culture. / Semithin sections of cells were embedded in Spurr epoxy resin and stained with methylene blue as shown: (A, B) untreated cells, (C, D) cells treated with 20 ng/mL TPA at 0 hour, (E, F) cells treated with TPA plus 5 ng/mL hr-NGF at 0 hour, and (G, H) cells treated with TPA plus 30 μg/mL anti-NGF Ab at 0 and 24 hours. (Original magnification ×40 in A, C, E, and G, and ×100 in B, D, F, and H. (C, D) Numerous cells showing aggregation of dense masses of chromatin beneath the nuclear membrane and nuclear fragmentation were observed. (E, F) Only a few cells showed morphological evidence of apoptosis, and (G, H) a high number of apoptotic cells were observed. Arrowheads indicate apoptotic bodies.

Light microscopy of BCBL-1 cells treated with TPA plus hr-NGF or anti-NGF Ab and observed at 48 hours of culture.

Semithin sections of cells were embedded in Spurr epoxy resin and stained with methylene blue as shown: (A, B) untreated cells, (C, D) cells treated with 20 ng/mL TPA at 0 hour, (E, F) cells treated with TPA plus 5 ng/mL hr-NGF at 0 hour, and (G, H) cells treated with TPA plus 30 μg/mL anti-NGF Ab at 0 and 24 hours. (Original magnification ×40 in A, C, E, and G, and ×100 in B, D, F, and H. (C, D) Numerous cells showing aggregation of dense masses of chromatin beneath the nuclear membrane and nuclear fragmentation were observed. (E, F) Only a few cells showed morphological evidence of apoptosis, and (G, H) a high number of apoptotic cells were observed. Arrowheads indicate apoptotic bodies.

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