Expression of high-affinity and low-affinity NGF receptors by HHV-8⁺ and HHV-8⁻ B-lymphoma cell lines.

The expression of high-affinity (p140^trk-A) and low-affinity (p75^NGFR) NGF receptors by B-lymphoma cell lines HHV-8⁺ (BC-1 and BCBL-1 cells) and HHV-8⁻ (RAMOS cells) is shown. Western blot analysis was performed, and immunostaining was completed with anti-p140 and anti-p75 antibodies of proteins (100 μg/sample) from untreated cells and BC-1 cells treated with 3 mmol/L sodium butyrate and BCBL-1 cells treated with 20 ng/mL TPA at 48 hours of culture.