CD43 (Alexa 488) and moesin (TRITC) double labeling of migrating neutrophils.

Cells, stimulated by fMLP, were allowed to locomote on fibronectin as described in “Materials and methods”. Cells were then fixed and permeabilized after 30, 60, or 140 seconds of locomotion. After washes and saturation steps, cells were double labeled for CD43 and moesin. A represents DIC and fluorescent images as indicated. B represents confocal images of CD43 (A, C) and of moesin (B, D). A and B represent a summation of the 19 planes of the stack; C and D represent a single plane from the middle of the stack. Scale bars = 10 μm.