Western blot analysis of IL-16 produced in DTH footpads.

Sensitized mice were challenged with mBSA into footpads, and swollen footpads were cut off, minced in cold PBS, and briefly incubated on ice. Resultant supernatant was immunoprecipitated by anti-IL-16 (14.1) or an isotype-matched control mAb (MOPS-173), electrophoresed on an SDS-polyacrylamide (10%-20% gradient) gel, and subjected to Western blot analysis using biotinylated anti-IL-16 (17.1) or isotype-matched control mAb (MOPC-21). Similar results were obtained in 2 independent experiments.