Kinetic analysis of the development of ABM CD34+Lin− cells in hybrid FTOC.

CD34+Lin− ABM cells were sorted and cultured together with mouse thymic lobes. After various incubation times (as indicated), the lobes were minced and a flow cytometric analysis was performed after staining the cells with monoclonal antibodies directed against human antigens as indicated in the axis of the dot plot. CyCD3, cytoplasmic CD3; mCD3, membrane CD3.