Fig. 6.
The ETS domain of PU.1 is required for interaction with GATA-1.
(A) The structure of the PUDC198 protein, in which most of the ETS domain has been deleted. (B) Wild-type PU.1 and PUDC198 were35S-labeled by in vitro translation, and 4-μL aliquots were separated on a 12% SDS-PAGE gel and detected by phosphorimaging. (C) Four-microliter 35S-labeled PU.1 (upper panel) and PUDC198 (lower panel) proteins were subjected to GST pulldown analysis and bound proteins detected as in Figure 5D.
