Evaluation of inter-assay reproducibility.

Inter-assay reproducibility is demonstrated by performing quantitative PCR on dilutions of NALM-6 cells on 4 separate occasions. Samples with ratios of NALM-6 to normal peripheral blood mononuclear cells ranging from 100% to 0.001% were quantified in triplicate on 4 successive days. IgH/GAPDH normalized values for the means of triplicate reactions of each sample on each day are plotted.