Evaluation of accuracy using ALL cell-line dilutions.

The ALL cell lines 697 and NALM-6 were diluted into normal peripheral blood mononuclear cells to produce samples that had tumor cell contamination values ranging from 100% to 0.001%. These samples were subjected to quantitative PCR in triplicate. Mean IgH/GAPDH normalized values with standard deviations are plotted with the expected values based on the amount of DNA added to the reactions.