Clonal deletion of T cells as a consequence of I-E transgene expression.

(A) Flow cytometric analysis of Vβ8, Vβ5, and Vβ11 expression among CD4⁺ and CD8⁺ LN T cells. LN cells of the 5 indicated mouse strains (n = 3 for B6-IE and B6 CD11c-IE; n = 6 for B6, B6 CD19-IE, B6 CD11c-CD19-IE) were triple stained with anti–CD4-PE, anti–CD8-CyChrome, and either anti-Vβ8.1,8.2-FITC, Vβ5.1, 5.2-FITC, or Vβ11-FITC, respectively. Results are expressed as a percentage of Vβ⁺CD4⁺ and Vβ⁺CD8⁺ cells. (B) Flow cytometric analysis of Vβ8, Vβ5, and Vβ11 expression among thymocytes. Total thymocytes were triple stained as described in (A). The percentages of positive cells among CD4⁺ or CD8⁺ thymocytes were determined (n = 3 for each type of transgenic mouse).