Flow cytometry analysis of nonadherent cells from bone marrow GM-CSF cultures derived from either nontransgenic mice (B6) or mice expressing transgenic MHC class II I-E under the control of the MHC class II promoter (B6-IE56) or the CD19 promoter (B6 CD19-IE).

Gates were set on large cells according to FSC/SSC criteria (top panel), and cells fulfilling these criteria were further analyzed for expression of the DC marker CD11c (PE) and MHC class II I-E (FITC).