![Fig. 6. Confocal imaging of 5 integrin in control and [Ca++]i-buffered PMNs plated on fibronectin. / PMNs were either loaded with quin2/AM (E-H) or not (A-D), then prepared for immunofluorescence as in Figure 3. Samples were incubated with 10 μg/mL of a monoclonal antibody to the extracellular domain of α5 integrin, rinsed with PBS, and then incubated with a TRITC-conjugated secondary antibody. The samples were viewed using a Bio-Rad MRC 600 laser scanning confocal microscope and vertical sections were obtained. x-y (A, E) and x-z (B, F) projections of both control (A-D) and quin2-buffered (E-H) cells are shown. Panels C and G show the localization of α5 at a single x-y slice at the depth indicated by the arrows in panels B and F, respectively. Panels D and H show a single x-z slice along the axis of the cells indicated by the arrows in panels A and E, respectively. Bar = 10 μm.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/8/10.1182_blood.v95.8.2471/4/bloo00726006y.jpeg?Expires=1724263214&Signature=yYvoLU7NZjoOZqnFPflrrGa7XUJE~KeJluYIajl00UE5w6dfBVzhfU2xHs0SkRvT8y0knsDAEkYPUQS6Lz8reEVgwbPMQa-73xx8LSWQCXn7G4W-3DXUdX2c8msPJ4JSkjG-eqQ7KLQS5eTkGELBdklsjz6cH-gmpbs7F6qoJXZ5HbPFdtolizBL5HZWc~FWYC3dHtyHe0~g7AwvLjeTy8KvZrKYfrIkbPrKzmrP6C5k-NdG2Qr-pyt9Mx6pLR6P1puBNfoL59ZaHwmVXq6coRVfOcdtJHe2vvCrDJBW~yKw6o0ZYFLP6rYPYCJO3WyUVyM4pqd7wmfR2HL~KkZV7w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Confocal imaging of 5 integrin in control and [Ca++]i-buffered PMNs plated on fibronectin.
PMNs were either loaded with quin2/AM (E-H) or not (A-D), then prepared for immunofluorescence as in Figure 3. Samples were incubated with 10 μg/mL of a monoclonal antibody to the extracellular domain of α5 integrin, rinsed with PBS, and then incubated with a TRITC-conjugated secondary antibody. The samples were viewed using a Bio-Rad MRC 600 laser scanning confocal microscope and vertical sections were obtained. x-y (A, E) and x-z (B, F) projections of both control (A-D) and quin2-buffered (E-H) cells are shown. Panels C and G show the localization of α5 at a single x-y slice at the depth indicated by the arrows in panels B and F, respectively. Panels D and H show a single x-z slice along the axis of the cells indicated by the arrows in panels A and E, respectively. Bar = 10 μm.
