![Fig. 1. Motility restoration of calcium-buffered neutrophils on fibronectin with polyclonal antibodies. / (A) Where indicated, cells were [Ca++]i-buffered by a 40 minutes' incubation in 50 μmol/L Quin2/AM. The cells were then incubated with a 1:2000 dilution of the indicated antisera, rinsed, plated on a fibronectin-coated coverslip dish, and stimulated with 10 nmol/L fMLP in the continued presence of antisera. (B) Motility restoration of calcium-buffered neutrophils on fibronectin with monoclonal antibodies. Where indicated, neutrophils were [Ca++]i-buffered with Quin2/AM as in (A), incubated with a 5 μg/mL solution of the indicated monoclonal IgG, rinsed, plated on a fibronectin-coated coverslip dish, and stimulated with 10 nmol/L fMLP in the continued presence of antibody. Cells able to move more than 7 μm in 200 seconds were considered motile. In each case, more than 150 cells were assayed. The data shown are mean values ± SEM.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/8/10.1182_blood.v95.8.2471/4/bloo00726001x.jpeg?Expires=1724260458&Signature=4wsU8RZdxH8hLx42BjXzjFFyYUuau~LmF457~JxebJ-sQLggJm0p8icWDa270KH9UYPpADk-AEbHM9Vwx6Fvb4aD9fit6dOTMgYSbQLI2x2Cytp6rzuPVag1bSpsqWpXAfAKhkWR2Z1gqyuiFP3X4W2Vs7oMi~9Z~yepRDNUJ8NG9MldO3-l8-8PX024DFNAbnigR3G2hyAmkg-hKe2voubdNRUNFbuAVDGUi1Zc2IFZ3tMbmkl2SjVHOhqIFdsu617aDH3aiCsAvq~FXeE0UuLLG70zOKs~ndK26t80bfqN7g634MlfJ7I6t1bo06cLJnAmfpFjiueEeKWPhNamVA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Motility restoration of calcium-buffered neutrophils on fibronectin with polyclonal antibodies.
(A) Where indicated, cells were [Ca++]i-buffered by a 40 minutes' incubation in 50 μmol/L Quin2/AM. The cells were then incubated with a 1:2000 dilution of the indicated antisera, rinsed, plated on a fibronectin-coated coverslip dish, and stimulated with 10 nmol/L fMLP in the continued presence of antisera. (B) Motility restoration of calcium-buffered neutrophils on fibronectin with monoclonal antibodies. Where indicated, neutrophils were [Ca++]i-buffered with Quin2/AM as in (A), incubated with a 5 μg/mL solution of the indicated monoclonal IgG, rinsed, plated on a fibronectin-coated coverslip dish, and stimulated with 10 nmol/L fMLP in the continued presence of antibody. Cells able to move more than 7 μm in 200 seconds were considered motile. In each case, more than 150 cells were assayed. The data shown are mean values ± SEM.
