Expression of G-CSF mRNA in PB CD4⁺ and CD8⁺ cells.

Purified CD4⁺ and CD8⁺ cells were derived by sorting PBMCs obtained from a normal healthy control. mRNA was extracted from the cells, and RT-PCR was performed using G-CSFR–specific primers. RT-PCR products were electrophoresed in agarose gel containing ethidium bromide and visualized under ultraviolet light. Unseparated normal bone marrow (nbm) from a normal control was used as a positive control.