Effects of G-CSF administration on IFN-γ and IL-4 production by peripheral blood lymphocytes.

Eleven normal stem cell donors received G-CSF (10 μg/kg per day) for 5 days. Blood was obtained before and after the administration of G-CSF. PBMC cells were purified by density-gradient centrifugation and were cultured with PHA for 48 hours. After permeabilization, cells were stained for IFN-γ and IL-4 as described in “Materials and methods.” Scattergrams represent log PE (CD4 or CD8) fluorescence activity versus log FITC (IFN-γ and IL-4) fluorescence activity for representative donors (A, B).