Effect of γ-radiation on cyclin B1 protein levels in Ramos cells.

(A) Western blots of protein lysates (10 μg/lane) were prepared at the indicated time points following treatment with 200 cGy of γ-radiation. Blots were probed with either cyclin A or cyclin B1 antibodies. (B) Western blot of protein lysates was prepared 4 hours following treatment with indicated doses of γ-radiation. Blots were probed with cyclin B antibodies. (C) DNA fragmentation was measured 24 hours following treatment with 200 cGy of γ-irradiation. Control cells were not irradiated but maintained under identical conditions. (D) Annexin V staining of cells 24 hours following treatment with 200 cGy of γ-radiation (white profile) relative to mock irradiated cells (black profile).