The phenotype of human PBMC-derived DCs.

DCs were generated by culturing adherent PBMCs of healthy donors for 7 days in the presence of GM-CSF and IL-4. Expression of the indicated antigens was analyzed by means of 1 color staining. Dead cells were excluded by gating out propidium-iodide–positive cells. Similar results were obtained by staining DCs from 3 unrelated donors.