Fig. 4.
![Fig. 4. ERK expression and activation in GM-CSF–treated TF-1 cells. / TF-1 cells were serum- and factor-starved for 24 hours, and placed in RPMI with BSA 0.5%. Cells were stimulated with rhGM-CSF (1 nmol/L) for 1, 2, 5, 10, and 20 minutes. TPA-stimulated cells represent the positive control and diluent-stimulated, the negative control (15-minute stimulation). The lysate from 2 × 105cells was separated on a 10% SDS-PAGE gel. Antiphosphorylated ERK antibody ([A], 1:20 000) or anti-ERK antibody ([B], 1:5000) was used to probe each blot. The blots represent samples taken from the same experiment loaded on 2 separate gels.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/95/8/10.1182_blood.v95.8.2552/4/bloo00830004w.jpeg?Expires=1724265106&Signature=MGcb3k-D9NRhqb34KYbDPsuF3sOKmi04y7rjDegEJJFA3JlUdcimDEmf81EWgvDxZRa72YWS9cO9VQzObx4iqib2Re9O52ZRe9mr1zpVL1qTae6t7xsQp4XRYMvJ8f9kDPvq~lD6GKGRIZ2LRaaxQDfqxMHDXM0M7MqONSF5RDSJKuHL39YJuZ3XVCeyFjuO8LYP0QTBgJeMgR4hC26EQQlIYx0FJnAdhy7jyRuA-vqaF5P-XfcI0ogupZkZjBOkt0HZR9GPee~3CcxiIkmrSomeetQ6bxHnTWSL3rM8qd~5PJ8zSR389kY17awWr3LtxlchhiC3dLpOZvfUPnOxlg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
ERK expression and activation in GM-CSF–treated TF-1 cells.
TF-1 cells were serum- and factor-starved for 24 hours, and placed in RPMI with BSA 0.5%. Cells were stimulated with rhGM-CSF (1 nmol/L) for 1, 2, 5, 10, and 20 minutes. TPA-stimulated cells represent the positive control and diluent-stimulated, the negative control (15-minute stimulation). The lysate from 2 × 105cells was separated on a 10% SDS-PAGE gel. Antiphosphorylated ERK antibody ([A], 1:20 000) or anti-ERK antibody ([B], 1:5000) was used to probe each blot. The blots represent samples taken from the same experiment loaded on 2 separate gels.
