Fig. 1.
Fig. 1. Expression of pp90RSK in TF-1 cells. / Unstimulated or GM-CSF–stimulated TF-1 cell nuclear (N) and cytoplasmic (C) extracts were isolated by a modified Dignam procedure.9 Protein (20 μg) was separated on a 10% polyacrylamide gel. The blot was probed with 3 μg/mL of pp90RSK antibody (UBI). ECL was used for detection. The arrow indicates pp90RSK protein. These results were confirmed in 2 independent experiments.

Expression of pp90RSK in TF-1 cells.

Unstimulated or GM-CSF–stimulated TF-1 cell nuclear (N) and cytoplasmic (C) extracts were isolated by a modified Dignam procedure.9 Protein (20 μg) was separated on a 10% polyacrylamide gel. The blot was probed with 3 μg/mL of pp90RSK antibody (UBI). ECL was used for detection. The arrow indicates pp90RSK protein. These results were confirmed in 2 independent experiments.

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