Expression of human F.IX in conditioned media of C2C12 murine myotubes after transduction with AAV vectors at an MOI = 105 vector genomes/cell.
All measurements of human F.IX concentrations were performed by ELISA. Transduction experiments were performed in duplicate. (A) Cells were transduced with either AAV-CMV-hF.IX-2 (CMV-2), AAV-CMV-hF.IX-3 (CMV-3), AAV-HSA-hF.IX-1 (HSA-1), or AAV-HSA-hF.IX-3 (HSA-3). F.IX values (ng/mL/24 hours) are average of 2 independent measurements performed on 24-hour supernatants at day 8 posttransduction. Vertical bars indicate standard deviation. (B) Cells were transduced with either AAV-CMV-hF.IX-3 (CMV-3), AAV-CMV-hF.IX-3-bGH (CMV-3-bGH), AAV-CMV-hF.IX-8 (CMV-8), AAV-HSA-hF.IX-3 (HSA-3), AAV-HSA-CMV-hF.IX-2 (HSA/CMV), or AAV-cTnT-hF.IX (cTnT). F.IX values (ng/mL/24 hours) are average of a total of 4 measurements (2 independent measurements per experiment) on 24-hour supernatants at day 8 posttransduction from 2 independent transduction experiments.
