PCR analysis of a Y-specific sequence in DNA extracted from peripheral blood of female IUT recipients who received at least 1 transfusion from a male donor.

Recipient numbers correspond to recipient numbers in Table 1. Lane 1 shows 100-bp size markers; lane 2 shows DNA from recipient 5; lane 3 shows DNA from recipient 8; lane 4 shows DNA from recipient 6; lane 5 shows DNA from recipient 3; lane 6 shows DNA from recipient 2; lane 7 shows DNA from recipient 7; lane 8 shows DNA from an IUT recipient not tested by the CTLp/HTLp assay; lane 9 shows DNA from a normal male; lane 10 shows a blank; lane 11 shows DNA from a nulligravid woman. The samples in lanes 3 to 9 show a band corresponding to the 237-bp product amplified from Y-chromosome DNA (indicated by the arrow).