Synergistic effect of VEGF on the BMP-dependent generation of CD34⁺ CD31⁺, Ter119⁺, and CD45⁺ EB cell population. E14 ES cells were induced to differentiate for 7 days in the serum-free medium without additional growth factor, with 20 ng/mL VEGF, with low concentration of BMP-4 (0.15 ng/mL), and with 0.15 ng/mL BMP-4 + 20 ng/mL VEGF. EB cells were harvested and stained with anti-CD45-PE, Ter119-PE or CD31-PE, and anti-CD34-FITC antibodies. Scatter patterns and isotype control staining patterns for EB cells are essentially the same as shown in Figure 1B. The designation of the regions is the same as in Figure 1. (left to right) R7: 0.0%, 0.05%, 0.22%, 3.5%; R3: 0.09%, 0.43%, 0.44%, 4.9%; R5: 0.25%, 0.71%, 1.0%, 3.5%; R6: 0.24%, 0.70%, 0.71%, 5.7%.