Western blot analysis of proteins interacting with the cytoplasmic tail of PSGL-1 in HL-60 cells.

HL-60 cell lysates were incubated with equivalent amounts of glutathione-Sepharose, glutathione S-transferase (GST), and GST-CyPSGL-1 fusion protein bound to glutathione-Sepharose 4B beads. Each immunoprecipitate was immunoblotted after 8% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with antimoesin 95/2 polyclonal antibody (pAb), antiezrin 90/3 pAb (A), or antivinculin mAb (B). Cell lysate blotted with antivinculin was included as the positive control.