Proliferative response to host alloantigen in mice with acute GVHD treated with anti-CD134L mAb.
Representative mice in each indicated group were killed on day 14 after BMT, and single-cell suspensions of spleen cells were prepared for responder cells in a mixed lymphocyte reaction. Responder cells were cultured with irradiated (30 Gy) stimulator DBA/2 (H-2d) or C3H/He (H-2k) spleen cells (2 × 105/well) in the presence or absence of IL-2 (200 U/mL) in 96-well round-bottomed microtiter plates. After 5 days, the cultures were pulsed for 18 hours with tritium thymidine and the incorporated radioactivity was measured in a β counter. Data are the mean ± SD results from triplicate samples. Responder cells from mice with acute GVHD were highly reactive against host alloantigen (H-2d), whereas responder cells from mice treated with anti-CD134L mAb showed little response to H-2d.
