NQ22 cells migrate more efficiently than NQ29 cells.

Transendothelial migration. H-end80 cells were grown to confluence on the upper side of uncoated wells of Transwell culture chambers and either were left untreated or were treated for 16 hours with 10 ng/mL TNF-α . NQ22 cells (•, ○) obtained from heavily infiltrated spleen and NQ29 cells from a subcutaneous mass (▪, □) (2 × 10⁵) were seeded in the upper chamber of the Transwell, and cells that migrated through untreated (○, □) or through TNF-α-treated (•, ▪) H-end80 cells to the lower chamber were harvested and counted at the indicated time-points. The values shown represent the mean of 3 optical fields.