Testosterone modulates AR expression in megakaryocytes.

CD34⁺ cells, grown in serum-free and phenol-red–free medium, were cultured in PEG-rhMGDF and 0 nmol/L, 10 nmol/L, or 100 nmol/L testosterone (T) as indicated for 13 days. Each experiment had a final concentration of 0.01% ethanol. Each row represents the same field of cells. Only approximately 10% of the total population of CD34-derived cells were negative for AR, suggesting that AR expression is widespread among CD34-derived cells. Staining with Dapi, GPIIb, and AR were as described in Materials aand methods. Original range of magnification for panels A through J: ×1200 to ×1400.