Localization of AR in human megakaryocytes using dual color immunofluorescence microscopy.

Similar to Figure 2 except that antisera specific for AR were used. (A) Negative control using mouse and rabbit IgGs as irrelevant primary antibodies. The same field of cells is shown in panels B through E. (B) Dapi staining of nucleic acid. (C) AR detected by specific rabbit polyclonal antisera C-19 (red). (D) GPIIb (green). (E) The co-localization of GPIIb and AR shown by summing the channels used in panels C and D. Panels F and G contain the same cell to emphasize the polyploid features of the megakaryocyte. (F) Dapi staining of nucleic acid. (G) Co-localization of GPIIb and the AR. Original magnification for panels B through E: ×1000. Original magnification for panels F and G: ×600.