▵716 cells are resistant to apoptosis induction following culture in G-CSF.

Ba/F3 cells transfected with the WT or Δ716 G-CSFR were grown in RPMI 1640 medium containing 10% FBS and 2 mmol/L glutamine, supplemented with either 10% WEHI-CM (IL-3 source) or 1.9 ng/mL G-CSF. At time 0, the cells were washed, resuspended in media without added cytokine, and incubated at 37°C. At the indicated times, cells (1 × 10⁵) were subjected to the TUNEL reaction and analyzed by light microscopy, as described in “Materials and methods.” Data are expressed as the percentage of non-apoptotic cells (negative for TUNEL reaction) in a 200-cell count from 2 independent experiments.