G-CSF pretreatment enhances the survival and proliferation of ▵716 cells following growth factor withdrawal.

Ba/F3 cells transfected with either the WT or the Δ716 G-CSFR were grown in media containing either 10% WEHI-CM (source of IL-3) or 1.9 ng/mL G-CSF. The cells were washed twice and then resuspended in RPMI 1640 containing 10% FBS and 2 mmol/L glutamine with no added cytokine. At the indicated times, cells were stained with trypan blue and cell number (A) and percent viability (B) were determined. SDs were calculated from 3 independent experiments.