Fig. 4.
Benzidine staining of K562 cells treated with butyrate in the presence of ERK inhibitor UO126 and p38 inhibitor SB203580, respectively.
Cells were cultured in the absence (control) or presence of butyrate alone (butyrate) or with butyrate plus UO126 (butyrate + UO126) and SB203580 (butyrate + SB203580), respectively. After harvesting, intracellular hemoglobin was detected by benzidine-staining and cell smears were subjected to microscopy using 100× magnification.
