A. Mutations in remaining allele of TP53.

Exons 4 through 8 of the TP53 gene were amplified by PCR using intronic primers and sequenced to identify mutations. Patients 1, 2, 3, 4, and ML3 each had a mutation in the coding region of the gene. B. Histograms of mutant TP53 alleles from patients. Point mutations are designated by arrows.