Detection of soluble FasL in the sera of C57BL/6+/+ tumor-bearing mice.

Sera from LSA or EL-4 tumor-bearing mice were pooled and used directly or after 1:2 dilution. Next, using ELISA, the presence of sFasL was detected. Control represents sera from C57BL/6 +/+ mice injected with PBS. The concentration of sFasL was calculated based on use of purified FasL as a standard.