Fig. 1.
Detection of FasL on LSA and EL-4 tumor cells grown in vivo.
Mice were injected intraperitoneally with 1 × 106tumor cells, and 7 days later they were killed and tumor cells were collected from the peritoneal cavity. Cells were analyzed for Fas ligand expression using RT-PCR (A) or flow cytometry (B). (A) Lane 1 is a molecular marker, and lanes 2 and 3 represent LSA and EL-4 tumor cells, respectively. β-actin served as an internal control. (B) Bold histogram represents negative control, and broken histogram represents cells stained with antibodies against FasL.
