Overexpression of Bcl-2 inhibits hexamethylene bisacetamide (HMBA)-mediated cell death.

(A) CEM and CEM-Bcl-2 cells were cultured with 10 mmol/L HMBA for 72 hours, and cell death was assayed by trypan blue exclusion. These data were calculated as the mean± SE of duplicate samples and are representative of at least three separate experiments. (B) After incubation with 0, 1, 5, 7.5, 10, and 15 mmol/L HMBA for 72 hours, cells were plated out in soft agar. Colonies were scored after 7 days in culture as described in “Materials and methods.” (C) Cytosolic lysates from CEM (lanes 1-2) or CEM-Bcl-2 (lanes 3-4) cells either untreated (lanes 1 and 3) or treated for 72 hours with 10 mmol/L HMBA (lanes 2 and 4) were separated by SDS/PAGE and Western blotted for cytochrome c (15-kD protein) and then α-Tubulin (50-kD protein).