Immunoprecipitation of tagged-δ1 expressed on Ba/F3 cells, under reducing conditions.

Ba/F3 cells transduced with tagged-δ1 and parenteral cells were surface-labeled with sulfo-NHS-LC-biotin, and the cell lysates were precipitated with anti-FLAG antibody followed by the blotting with streptavidin-horseradish peroxidase. Positions of δ1-FLAG (arrowhead) and molecular weight markers are indicated.