Fig. 5.
Activation of JAK2 kinase through the ectopically expressed chimeric EDE receptor in FDC-P1 cells.
The ability of the chimeric receptor EDER to mediate hEPO- dependent activation of JAK kinases was assayed by immunoprecipitation with antibody to JAK2 and Western blotting with antibody to phosphotyrosine (monoclonal antibody 4G10). Reprobing was carried out using the anti-JAK2 antibody after stripping. Similar analyses were also performed using anti-JAK1, anti-JAK3, and anti-TYK2 antibodies.
