Fig. 3.
Pulse-chase analysis of rvWF C788R and rvWF C1225G expressed in COS-7 cells.
COS-7 cells were transfected with the appropriate plasmid DNA. At 48 hours posttransfection, the cells were radiolabeled for 20 minutes with35S-methionine and 35S-cysteine and then chased for various times in the presence of nonradioactive methionine and cysteine. Radiolabeled cell lysates and medium samples were immunoprecipitated with a rabbit polyclonal antiserum to vWF. Immunoprecipitates were separated by electrophoresis on 6% reducing SDS-polyacrylamide gels. Upper bar denotes the position of pro-vWF; lower bar denotes the position of mature propeptide cleaved vWF.
