American Society of Hematology

Fig. 2.

$Fig. 2. Steady-state analysis of rvWF C788R, rvWF C1225G, and mutant/wild-type hybrids expressed in COS-7 cells. / The cells were transfected with the appropriate plasmid DNA, then placed in serum-free medium 24 hours posttransfection. Cell lysates and medium supernatants were harvested after an additional 48 hours, and the quantities of vWF in each fraction were measured by ELISA. Each bar represents the average values with standard deviations of 4 transfections, and these values are expressed as percentages relative to wild-type vWF in 1 mL of lysate (31 mU ± 1 mU, n = 4) or 5 mL of medium (70 mU ± 5 mU, n = 4) of each transfection.$

Steady-state analysis of rvWF C788R, rvWF C1225G, and mutant/wild-type hybrids expressed in COS-7 cells.

The cells were transfected with the appropriate plasmid DNA, then placed in serum-free medium 24 hours posttransfection. Cell lysates and medium supernatants were harvested after an additional 48 hours, and the quantities of vWF in each fraction were measured by ELISA. Each bar represents the average values with standard deviations of 4 transfections, and these values are expressed as percentages relative to wild-type vWF in 1 mL of lysate (31 mU ± 1 mU, n = 4) or 5 mL of medium (70 mU ± 5 mU, n = 4) of each transfection.

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