Fig. 6.
Northern blot analysis of regulation of protein S messenger RNA expression by thrombin.
Human vascular smooth muscle cells (HVSMCs) at 90% confluency were incubated with α-thrombin (5 U/mL) for the indicated times. Total RNA was electrophoresed on 1.2% agarose gel, transferred to a nylon membrane, and sequentially hybridized with 32P-labeled protein S and G6PD complementary DNA probes.
