Increase in the percentage of CD44^hi T lymphocytes in the CD4+ and CD8+ T cells from spleens and lymph nodes of mice treated with CTX or poly (I:C).

Seven- to 8-week-old DBA/2 mice were injected intraperitoneally with CTX (83 mg/kg) (dotted bars), poly (I:C) (0.15 mg/mouse) (striped bars), or saline (white bars). At different time intervals, spleens and a pool of lymph nodes were taken and disaggregated. Cells were stained for CD44 and for CD4 or CD8 surface antigen expression before being processed for cytofluorimetric analysis. There were 3 mice per group. The data show the mean (± SE) value of the percentage of CD44^hi cells on CD4 (left panels) or CD8 (right panels) lymphocytes, in the spleen (upper panels) or in the pooled lymph nodes (lower panels).