Effect of 2β1 antibody cross-linking on AICD.

Jurkat cells were cultured for 24 hours in wells that had been coated with anti-CD3 and with 50 μg/mL of activating anti-α2β1 antibody JSB2 or with control antibodies (IgG). 50 μg/mL of poly-l-lysine or collagen I was added to some of the wells as indicated in the figure. DNA fragmentation was determined as previously described.