Effects of ▵STAT5 on viability of Ba/F3p210 cells.

Cells from 3 clones of Ba/F3p210ΔSTAT5 cells were maintained in either the presence or absence of doxycycline (1 μg/mL) for 2 days, and viability was determined by using a combined annexin V/PI staining protocol.29 30 Compared with cells not subjected to induction (black bars), ΔSTAT5 expression induced by doxycycline (stippled bars) increased the percentage of annexin V-positive cells (apoptotic cells) in all clones tested. Doxycycline had no effect on the viability of Ba/F3p210Ctrl cells.