Genomic Southern hybridization with the ETV6probes.

(A) With the full length ETV6 cDNA probe, additional bands were found only in the HT93A cells, but not in control cells. The additional bands on Bam HI, EcoRI, and SacI digests were hybridized with A-I probe and A-II probe, respectively. (B) Schematic representation of genomic breakpoint in the HT93A cells. Taking into account the restriction map of ETV6, the breakpoint is deduced to localize in a 1780 bp region that encompasses intron 5 and exon 6 ofETV6.