Fig. 4.
Expression analysis of candidate genes/ESTs.
RT-PCR was performed on human bone marrow CD34+ cells and myeloid cell lines, AML-193, KG-1, and HL-60, as indicated, and was visualized by agarose gel electrophoresis and ethidium bromide staining. The left column lists the gene or EST that was tested, using the PCR primers indicated in Table 2. The right column indicates the product size in base pairs. The PCR results are displayed for each primer set, with or without the addition of RT, indicated by (+) or (−), respectively. Negative controls for template pairs (no RNA added) is not shown. β2-Microglobulin primers were used as the positive control for RT-PCR.
