Early pre-B cells are depleted in estrogen-treated BALB/c mice.

(A) B-lineage populations in bone marrow from control and E2-treated mice were revealed by surface staining with antibodies to CD45R and CD43. Mature B cells were identified as CD45R^hiCD43⁻. (B) Cells within the CD45R⁺CD43⁺ gate were then analyzed for cμ heavy chains (solid lines) as compared with staining with a normal goat IgG control (dotted lines). The histograms depict 50 000 events collected within lymphocyte light scatter gates and are representative of results obtained from 7 individual control and 9 estrogen-treated mice.