American Society of Hematology

Fig. 7.

$Fig. 7. Northern blot analysis of Ang-1 transcripts in CHRF cells. / Polyadenylated RNA (2 μg) was fractionated on a 1% agarose gel and transferred to nylon membranes. Membranes were hybridized with either probe 1,1.5 kb full-length Ang-1 or probe 2 (approximately 0.5 kb of the alternatively spliced part of Ang-1, which is absent in the 0.9-kb and 0.7-kb isoforms), or probe 3 (0.17 kb of the alternatively spliced part of Ang-1, which is absent in the 1.3-kb and 0.7-kb isoforms). A schematic diagram of the probes is shown in the upper panel. The thin lines represent the alternatively spliced parts of Ang-1 that gave rise to the 1.3-, 0.9-, and 0.7-kb isoforms. Lane 1, result from hybridization with probe 1, which detected all 4 mRNA isoforms; lane 2, hybridization with probe 2, which detected the 1.5-kb and 1.3-kb isoforms; lane 3, hybridization with probe 3, which detected the 1.5-kb and 0.9-kb isoforms. Sizes of RNA markers are given on the right.$

Northern blot analysis of Ang-1 transcripts in CHRF cells.

Polyadenylated RNA (2 μg) was fractionated on a 1% agarose gel and transferred to nylon membranes. Membranes were hybridized with either probe 1,1.5 kb full-length Ang-1 or probe 2 (approximately 0.5 kb of the alternatively spliced part of Ang-1, which is absent in the 0.9-kb and 0.7-kb isoforms), or probe 3 (0.17 kb of the alternatively spliced part of Ang-1, which is absent in the 1.3-kb and 0.7-kb isoforms). A schematic diagram of the probes is shown in the upper panel. The thin lines represent the alternatively spliced parts of Ang-1 that gave rise to the 1.3-, 0.9-, and 0.7-kb isoforms. Lane 1, result from hybridization with probe 1, which detected all 4 mRNA isoforms; lane 2, hybridization with probe 2, which detected the 1.5-kb and 1.3-kb isoforms; lane 3, hybridization with probe 3, which detected the 1.5-kb and 0.9-kb isoforms. Sizes of RNA markers are given on the right.

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