Interleukin 3 (IL-3)-induced immunoglobulin A (IgA) binding is inhibited by the phosphatidylinositol 3-kinase (PI3K) inhibitors, wortmannin and LY294002.

Cytokine-starved Ba/F3_FcαR cells were pretreated for 15 minutes at 37°C with buffer, 50 μmol/L MEK inhibitor PD98059, 1 μmol/L p38 MAPK inhibitor SB203580, 20 nmol/L wortmannin, or 1 μmol/L LY294002, and subsequently stimulated at 37°C with buffer (white bars) or IL-3 (1:1000; gray bars) for 15 minutes. Binding of IgA beads to these cells was measured, and results are expressed as rosette index (number of beads/100 cells) and as means ± SE (n = 3).