Fig. 3.
Effect of interleukin 3 (IL-3) stimulation on the phosphorylation of ERK1/2, p38 MAPK, and protein kinase B (PKB) in Ba/F3 cells.
Cytokine-starved Ba/F3 cells were stimulated with IL-3 for the indicated time. After stimulation, cells (0.2 × 106per sample) were washed with ice-cold phosphate-buffered saline, lyzed in lysis buffer, and heated for 5 minutes after addition of 5x sample buffer. Phosphorylation of ERK1/2 (A), p38 MAPK (B), and PKB (C) was detected, using polyclonal anti-phospho-ERK1/2, anti-phospho-p38, or anti-phospho-PKB antiserum for Western blotting.
