PECAM/CD34 double-label immunofluorescent antibody staining of hemizygous VEGF mutant ES cultures.

Day 8 VEGF hemizygous (+/−) ES cultures were fixed and stained with rat antimouse CD34 (red) and rabbit polyclonal antimouse PECAM (green) antibodies, along with the appropriate secondary antibodies. Images were photographed with the appropriate FITC (B) or rhodamine (C) filters or with a double exposure using both filters sequentially (A). The arrow in each frame points to a clump of cells that stained with the PECAM antibody but not with the CD34 antibody. The arrowheads in each frame point to several individual cells that stained with the CD34 antibody but not with the PECAM antibody. Magnification, 50×.